The trail is contiguous with the Camp Milton Historic Preserve, which serves as a mid-point trailhead. Five other trailheads along the Rail Trail are located at Imeson Road, Halsema Road, Otis Road, the old trail depot in the City of Baldwin, and Brandy Branch Road. There are approx. 8.5 miles of spur trails onto the Jacksonville Rail Trail Buffer Properties that are designated for unpaved day hiking and equestrian use.
Trailheads are located at the following locations:
Imeson Road - 1800 Imeson Road, 32220
Camp Milton Historic Preserve - 1225 Halsema Road, 32220
Livestock Farm - 1172 Halsema Road, 32220
Otis Raod - 1380 Otis Road, 32220
Baldwin - 850 Center Street North, 32234
Brandy Branch Road - 89 Brandy Branch Road, 32234